추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
EA10, monoclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
human
반응하면 안 됨
mouse, rat
제조업체/상표
Calbiochem®
저장 조건
do not freeze
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... CDKN1A(1026)
일반 설명
Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
This Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) is validated for use in FC, Immunoblotting, IF, IP, Paraffin Sections for the detection of p21WAF1 (Ab-1).
애플리케이션
Flow Cytometry (2 g/ml or use Cat. No. OP64F; see application references)
Frozen Sections (5 g/ml or use Cat. No. OP64F)
Immunoblotting (1-3 g/ml)
Immunofluorescence (1-5 g/ml or use Cat. No. OP64F)
Immunoprecipitation (2 g/sample)
Paraffin Sections (5 g/ml or use OP64F; heat pre-treatment required; see comments and application references)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
분석 메모
Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
기타 정보
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. This antibody will immunoprecipitate p21WAF1 but not associated proteins. For immunoblotting applications, use a 0.22 µm filter and visualize by chemiluminescence. For staining paraffin sections, heating the tissue in 10 mM citrate buffer is required (see application references). In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. Antibody should be titrated for optimal results in individual systems.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Mingxuan Xia et al.
Cell cycle (Georgetown, Tex.), 7(11), 1604-1612 (2008-06-04)
The p53 tumor suppressor is a powerful growth suppressive and pro-apoptotic molecule frequently inactivated in human cancer. Many tumors overproduce its negative regulator MDM2, a specific p53 ubiquitin ligase and transcriptional inhibitor, to disable p53 function. Therefore, p53 activation by
Vladimir A Shamanin et al.
Molecular and cellular biology, 24(5), 2144-2152 (2004-02-18)
Inactivation of the ARF-p53 tumor suppressor pathway leads to immortalization of murine fibroblasts. The role of this pathway in immortalization of human epithelial cells is not clear. We analyzed the functionality of the p14(ARF)-p53 pathway in human mammary epithelial cells
Yuki Minagawa et al.
Anticancer research, 40(10), 5631-5639 (2020-09-30)
DNA damage response (DDR), wherein p21 is a cell fate determinant, is a potential cancer therapeutic target. Molecular expression during DDR was explored in ovarian clear-cell carcinoma (CCC). CHK1, CHK2, TP53 and p21 expression in DDR was examined using immunostaining
I Osen et al.
British journal of urology, 81(6), 862-869 (1998-07-17)
To determine the prognostic role of p53, Ki-67 and p21 for patients with muscle-invasive bladder cancer treated with curative intent by radiotherapy. The study included 131 patients (24 women and 107 men, median age 72 years, range 40-86) with transitional
R Røtterud et al.
Acta oncologica (Stockholm, Sweden), 40(5), 644-652 (2001-10-24)
The aim of this study was to examine any relation between DNA ploidy and previously detected TP53 (p53) or p21WAF1/CIP1 expression in 94 patients with muscle-invasive transitional cell carcinoma of the urinary bladder and to associate these factors with survival.
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