추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
porcine, human, rat, mouse
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... UCHL1(7345)
일반 설명
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.
Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.
This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).
면역원
a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
애플리케이션
Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)
경고
Toxicity: Standard Handling (A)
물리적 형태
Undiluted serum.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
Mouse dorsal root nerve fibers
Mouse dorsal root nerve fibers
기타 정보
Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
PGP9.5 is also overexpressed in some cancers.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
J Nakano et al.
Physiological research, 61(6), 643-647 (2012-10-27)
This study was designed to investigate histological changes in skin tissue accompanying immobilization-induced hypersensitivity. Changes in mechanical sensitivity, epidermal thickness, and peripheral nerve profiles in the upper dermis were examined in glabrous skin of rat hind paw after 1, 2
Masato Ogura et al.
PloS one, 16(2), e0246630-e0246630 (2021-02-05)
Interleukin-31 (IL-31) is involved in excessive development of cutaneous sensory nerves in atopic dermatitis (AD), leading to severe pruritus. We previously reported that PQA-18, a prenylated quinolinecarboxylic acid (PQA) derivative, is an immunosuppressant with inhibition of p21-activated kinase 2 (PAK2)
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