생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
14PO2, monoclonal
양식
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
human
반응하면 안 됨
rat, mouse
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG1
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
human ... CDKN2A(1029)
일반 설명
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
This Anti-p14ARF (Ab-2) Mouse mAb (14PO2) is validated for use in Immnunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p14ARF (Ab-2).
면역원
Human
recombinant, human p14ARF (p16β)
애플리케이션
Immnunoblotting (2-4 µg/ml)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 10 mM PBS, 0.2% BSA, pH 7.4.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Negative Control
Normal sheep serum
Normal sheep serum
Positive Control
HeLa cells
HeLa cells
기타 정보
Antibody should be titrated for optimal results in individual systems.
Bates, S., et al. 1998. Nature395, 124.
법적 정보
Sold under license of U.S. Patent 6,482,929.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Cancer cell, 1(4), 393-401 (2002-06-28)
Ewing's sarcoma is associated with a fusion between the EWS and FLI1 genes, forming an EWS/FLI fusion protein. We developed a system for the identification of cooperative mutations in this tumor through expression of EWS/FLI in primary human fibroblasts. Gene
FEBS letters, 586(4), 435-441 (2012-01-31)
ARF is the second most commonly inactivated tumor suppressor behind p53. It has been implicated in the control of cell proliferation, cell senescence, and tumor suppression. However, the detailed mechanism underlying the transcriptional control of ARF remains largely unknown. Here
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