추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
PC10, monoclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
rat, yeast, mouse, human, zebrafish
제조업체/상표
Calbiochem®
저장 조건
do not freeze
동형
IgG2a
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... PCNA(5111)
zebrafish ... Pcna(30678)
일반 설명
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).
면역원
Epitope: Within amino acids 112-121
recombinant PCNA
애플리케이션
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
분석 메모
Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
기타 정보
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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