MAC140

Anti-Inntag 10 (IT10) Antibody, clone IT10

clone IT10-1, from mouse

• 동의어(들): Allergen Amb t V, Allergen Ra5G, Amb t 5
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: IT10-1, monoclonal
• 종 반응성: Ambrosia trifida
• 종 반응성(상동성에 의해 예측): all
• 포장: antibody small pack of 25 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• UniProt 수납 번호: P10414
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Pollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag IT10 is based on a packed beta-sheet conformation.

특이성

Clone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.

면역원

His-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.

애플리케이션

Anti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected Inntag 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunocytochemistry Analysis: A representative lot detected Inntag 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

품질

Evaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 10 (IT10) in 10 µg of transfected HEK293/GFP-IT10 cell lysates.

표적 설명

~33.7 kDa observed; 8.06 kDa calculated. 33.7 kD observed corresponds to 29.4 kDa GFP plus 4.3 kDa IT10. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable