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Merck
모든 사진(4)

Key Documents

MAC133

Sigma-Aldrich

Anti-Cas9 Antibody, clone 7A9

clone 7A9, from mouse

동의어(들):

dCas9

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

7A9, monoclonal

종 반응성(상동성에 의해 예측)

all

기술

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1κ

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3′-5′ exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.

특이성

MAC133 recognises both Cas9 and dCas9 (nuclease deficient Cas9)

면역원

N-Terminal domain of Cas9.

애플리케이션

Anti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).

품질

Evaluated by Western Blotting in HEK293 expressing Flag-Cas9 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.

표적 설명

~160 kDa observed

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Maor Sheva et al.
Frontiers in plant science, 11, 607174-607174 (2020-12-17)
While plant cells in suspension are becoming a popular platform for expressing biotherapeutic proteins, the need to pre-engineer these cells to better comply with their role as host cell lines is emerging. Heterologous DNA and selectable markers are used for
Guo-Xiang Ruan et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(2), 331-341 (2017-01-23)
As the most common subtype of Leber congenital amaurosis (LCA), LCA10 is a severe retinal dystrophy caused by mutations in the CEP290 gene. The most frequent mutation found in patients with LCA10 is a deep intronic mutation in CEP290 that
Matija Snuderl et al.
Nature communications, 9(1), 2868-2868 (2018-07-22)
Pineoblastoma is a rare and highly aggressive brain cancer of childhood, histologically belonging to the spectrum of primitive neuroectodermal tumors. Patients with germline mutations in DICER1, a ribonuclease involved in microRNA processing, have increased risk of pineoblastoma, but genetic drivers
Garmen Yuen et al.
Nucleic acids research, 45(20), 12039-12053 (2017-10-17)
CRISPR/Cas9 is a powerful gene editing tool for gene knockout studies and functional genomic screens. Successful implementation of CRISPR often requires Cas9 to elicit efficient target knockout in a population of cells. In this study, we investigated the role of
Elif Eren et al.
EMBO reports, 21(11), e50829-e50829 (2020-10-31)
Inflammatory caspase-11 (rodent) and caspases-4/5 (humans) detect the Gram-negative bacterial component LPS within the host cell cytosol, promoting activation of the non-canonical inflammasome. Although non-canonical inflammasome-induced pyroptosis and IL-1-related cytokine release are crucial to mount an efficient immune response against

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