MABT850M

Anti-Podoplanin Antibody, clone PMab-2

clone PMab-2, from mouse

• 동의어(들): E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PMab-2, monoclonal
• 종 반응성: rat
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• UniProt 수납 번호: Q64294
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Podoplanin (UniProt: Q64294; also known as E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein) is encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 54320) in rat. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-135), a helical domain (aa 136-156) and a cytoplasmic region (aa 157-166). Podoplanin is extensively O-glycosylated and contains sialic acid residues. O-glycosylation is believed to be essential for its platelet aggregation activity. Clone PMab2 exhibits high affinity for podoplanin (Kd = 0.59 nM). (Ref.: Oki, H et al. (2015). Monoclon. Antib. Immunodiagn. Immunother. 34(6); 396-403).

특이성

Clone PMab-2 detects podoplanin in rat spleen and kidney. It targets an epitope within 14 amino acids from the N-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of rat Podopalnin.

애플리케이션

Anti-Podoplanin, clone PMab-2, Cat. No. MABT850, is a mouse monoclonal that detects rat podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in rat spleen tissue.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

품질

Evaluated by Immunohistochemistry in rat kidney tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in rat kidney tissue sections.

표적 설명

17.58 kDa calculated.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1