MABT1562

Anti-CFAP43 Antibody, clone 27D5

• 동의어(들): Cilia- and flagella-associated protein 43
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 27D5, monoclonal
• 분자량: calculated mol wt 193.43 kDa; observed mol wt ~190 kDa
• 정제법: using protein G
• 종 반응성: mouse
• 포장: antibody small pack of 100 μL
• 기술: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2b
• 에피토프 서열: C-terminal half
• 단백질 ID 수납 번호: NP_081835
• UniProt 수납 번호: E9Q7R9
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Cfap43(951475)

설명

일반 설명

Cilia- and flagella-associated protein 43 (UniProt: E9Q7R9; also known as CFAP43) is encoded by the Cfap43 gene (Gene ID: 100048534) in murine species. CFAP43, a flagellar protein with six WD repeats, is expressed in testis, lung, brain, oviduct, and nasal cavity. It is detected very early during embryonic development It is involved in the regulation of the beating frequency of motile cilia on the epithelial cells. It is also involved in the formation of flagellar axoneme organization and function during spermatogenesis. Mutations in Cfap43 gene have been linked to male infertility. Any malfunction of motile cilia, resulting from defective CFAP43, cause a variety of developmental defects in humans and animal models. These defects may also include impaired mucociliary clearance of airways and hydrocephalus. Cfap43 knockout mice (-/-) exhibit dilation of the third and lateral ventricles. (Ref.: Rachev, E. (2020). Dev. Biol. 459(2); 109-125; Morimoto, Y., et al. (2019). Neurology. 92(20); e2364-e2374; Coutton, C., et al. (2018). Nature Commun. 9; Article 686).

특이성

Clone 27D5 is a mouse monoclonal antibody that detects Cilia- and flagella-associated protein 43. It targets an epitope within the C-terminal half.

면역원

MBP-tagged recombinant fragment corresponding to 174 amino acids from the C-terminal half of mouse Cilia- and flagella-associated protein 43 (CFAP43).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue extracts.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP43 in Mouse testis tissue extracts.

Tested Applications

Western Blotting Analysis: A representative lot detected CFAP43 in Western Blotting applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunofluorescence Analysis: A representative lot detected CFAP43 in Immunofluorescence applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CFAP43 in Immunoprecipitation applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

물리적 형태

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable