추천 제품
생물학적 소스
mouse
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
10B2, monoclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
포장
antibody small pack of 25 μg
기술
immunofluorescence: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
human ... PKP1(5317)
일반 설명
Plakophilin-1 (UniProt: Q13835; also known as Band 6 protein, B6P) is encoded by the PKP1 gene (Gene ID: 5317) in human. Plakophilins are proteins that are involved in linking adherins to cytoskeletal intermediate filaments. They contain numerous armadillo (ARM) repeats and localize to cell desmosomes and nuclei. Plakophilin-1 may be involved in molecular recruitment and stabilization during desmosome formation. It is also shown to be important for tooth development and regulates ZO-1 distribution in the dental epithelium. Two isoforms of Plankophilin-1 have been reported that are produced by alternative splicing. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia and isoform 2 is widely expressed. Plakophilin-1 is shown to be highly expressed in teeth and skin, and was upregulated during tooth development. It is reported to be involved in the regulation of Wnt signaling via its ARM repeat domains in a manner similar to beta-catenin and Wnt activation induces its nuclear translocation. Mutations n PKP1 gene are shown to cause ectodermal dysplasia-skin fragility syndrome that is characterized by cutaneous fragility and congenital ectodermal dysplasia affecting skin, hair and nails. (Ref.: Sprecher, E., et al (2004). J. Invest. Dermatol. 122(3); 647-51; Miyazaki, K et al. (2016). PLoS ONE 11(3): e0152206).
특이성
Clone 10B2 specifically detects Planophilin-1 and targets an epitope with in the first 27 amino acids from the N-terminal region.
면역원
Epitope: N-terminus
MBP-conjugated recombinant fragment corresponding to the first 235 amino acids from the N-terminal region of human Plakophilin-1.
애플리케이션
Anti-Plakophilin 1, clone 10B2, Cat. No. MABT1505, is a mouse monoclonal antibody that detects Plantophilin 1 and has been tested for use in Immunofluorescence and Western Blotting.
Research Category
Cell Structure
Cell Structure
Western Blotting Analysis: A representative lot detected Plakophilin 1 in Western Blotting applications (Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
Immunofluorescence Analysis: A representative lot detected Plakophilin 1 in Immunofluorescence applications (Sprecher, E., et. al. (2004). J Invest Dermatol. 122(3):647-51;Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
Immunofluorescence Analysis: A representative lot detected Plakophilin 1 in Immunofluorescence applications (Sprecher, E., et. al. (2004). J Invest Dermatol. 122(3):647-51;Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
품질
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin 1 in 10 µg of PC3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin 1 in 10 µg of PC3 cell lysate.
표적 설명
~80 kDa observed; 80.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
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