콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

MABT1351

Sigma-Aldrich

Anti-Tspan8-C Antibody, clone 51/15-8G4-17-42-1

clone 51/15-8G4-17-42-1, from rat

동의어(들):

Tetraspanin-8, Tspan-8

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

51/15-8G4-17-42-1, monoclonal

종 반응성

human, mouse

포장

antibody small pack of 25 μL

기술

immunohistochemistry: suitable
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Tspan8(216350)

일반 설명

Tetraspanin-8 (UniProt: Q8R3G9; also known as Tspan8, Tspan-8) is encoded by the Tspan8 gene (Gene ID: 216350) in murine species. Tspan-8 is a multi-pass membrane protein of the tetraspanin family that is involved in the regulation of cell activation, growth, and motility. Members of this family have emerged as important regulators of expression levels, trafficking or post-translational modification of laminin-binding integrins. Tetraspanins are shown to associate with one another and cluster dynamically with a large variety of transmembrane and signal-transducing partners, forming specialized membrane microdomains called tetraspanin web. Tspan-8 is reported to be overexpressed during the progression of colorectal, liver, pancreatic and gastric cancers and its increased expression is shown to promote liver and lung metastasis. Overexpression of Tspan-8 has also been correlated with poor prognosis in human colorectal cancer. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tspan-8 and Tspan CD151 with integrin 6 4 and subsequent internalization of this integrin-tetraspanin complex, resulting in decreased cell-matrix adhesion and increased cell migration. Tspan-8 knockdown in HT29 colon cancer cells is reported to significantly reduce their migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. (Ref.: Kharbili, ME., et al. (2017). Oncotarget. 8(10); 17140-17155).

특이성

Clone 51/15-8G4-17-42-1 is a rat monoclonal antibody that detects human and murine Tetraspanin-8. It targets an epitope with in 12 amino acids from the extracellular domain.

면역원

Epitope: extracellular domain
KLH-conjugated linear peptide corresponding to 12 amino acids from the extracellular domain of mouse Tetraspanin-8.

애플리케이션

Anti-Tspan8-C, clone 51/15-8G4-17-42-1, Cat. No. MABT1351, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Tspan8-C in Immunohistochemistry applications (Fu, N.Y., et. al. (2017). Nat Cell Biol. 19(3):164-176).
Research Category
Cell Structure

품질

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected Tspan8-C in HT-29 cell lysate.

표적 설명

~25 kDa observed; 25.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Nai Yang Fu et al.
Nature cell biology, 19(3), 164-176 (2017-02-14)
Despite accumulating evidence for a mammary differentiation hierarchy, the basal compartment comprising stem cells remains poorly characterized. Through gene expression profiling of Lgr5+ basal epithelial cells, we identify a new marker, Tetraspanin8 (Tspan8). Fractionation based on Tspan8 and Lgr5 expression

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.