MABS2773

Anti-c-Kit/CD117 Antibody, clone H2C7

• 동의어(들): CD117, EC:2.7.10.1, Mast/stem cell growth factor receptor Kit, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: hamster
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: H2C7, monoclonal
• 분자량: observed mol wt ~N/A kDa
• 정제법: using protein G
• 종 반응성: mouse
• 포장: antibody small pack of 100
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable
• 동형: IgG
• 에피토프 서열: Extracellular domain
• 단백질 ID 수납 번호: NP_001116205
• UniProt 수납 번호: P05532
• 저장 온도: 2-8°C
• 유전자 정보: mouse ... Kit(16590)

설명

특이성

Clone H2C7 is an Armenian hamster monoclonal antibody that detects c-Kit/CD117. It targets an epitope within the extracellular domain.

면역원

E14 mouse olfactory cortices.

애플리케이션

Quality Control Testing

Evaluated by Immunohistochemistry in E14 Mouse liver sections.

Immunohistochemistry Applications: A 1:25 dilution from a representative lot detected c-kit/CD117 in E14 Mouse liver frozen tissue sections.

Tested Applications

Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected c-Kit/CD117 in developing mouse forebrain frozen tissue sections.

Immunocytochemistry Analysis: A representative lot detected c-Kit/CD117 in Immunocytochemistry applications (Koma, Y., et al. (2005). Lab Invest. 85(3):426-35).

Immunohistochemistry Applications: A representative lot detected c-Kit/CD117 in Immunohistochemistry applications (Yamatani, H., et al. (2004). J Comp Neurol. 475(2):247-60; Takagi, K., et al. (2008). Neuroscience. 153(4):1278-88).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Mast/stem cell growth factor receptor Kit (UniProt: P05532; also known as EC:2.7.10.1, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117) is encoded by the Kit (also known as SI) gene (Gene ID: 16590) in murine species. c-Kit is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-527), a transmembrane domain (aa 528-548), and a cytoplasmic domain (aa 549-979). c-Kit has tyrosine protein kinase activity and serves as a cell-surface receptor for the stem cell factor (SCF)/KITLG. It plays a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis, and other functions. In response to SCF binding, c-Kit can activate several signaling pathways. In the absence of bound SCF, it exists as a monomer, however, it homodimerizes in the presence of bound SCF and forms a heterotetramer with two SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and SCF binding enhances its autophosphorylation. Its kinase activity is reported to be down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at tyrosine 571 is shown to be essential for its interaction with PTPN11/SHP-2 and phosphorylation of tyrosine 573 is required for interaction with PTPN6/SHP-1. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by SCF binding, leading to its internalization and degradation. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy. c-kit expression is also reported in peptidergic small-sized dorsal root ganglia neurons and is involved peripherally and centrally in pain regulation. (Ref.: Foster, BM., et al. (2018). Biomedicines. 6(1); 31; Takagi, K., et al. (2008). Neuroscience. 153(4); 1278-1288; Koma, Y., et al. (2005). Lab. Invest. 85; 426-435; Yee, NS., et al. (1994). J. Biol. Chem. 269(50); 1991-1998).

물리적 형태

Purified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable