MABS2219

Anti-MGAT1 Antibody, clone 5F5

• 동의어(들): Anti-MGAT1 Antibody, clone 5F5
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 5F5, monoclonal
• 분자량: calculated mol wt 38.79 kDa; observed mol wt ~36 kDa
• 정제법: using protein G
• 종 반응성: mouse
• 포장: antibody small pack of 100 μg
• 기술: western blot: suitable
• 동형: IgG2κ
• 에피토프 서열: C-terminal half
• 단백질 ID 수납 번호: NP_080989
• UniProt 수납 번호: Q91ZV4
• 배송 상태: dry ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Mogat1(68393)

설명

일반 설명

2-acylglycerol O-acyltransferase 1 (UniProt: EC:2.3.1.22, Acyl-CoA:monoacylglycerol acyltransferase 1, MGAT1,Diacylglycerol acyltransferase 2-like protein 1, Monoacylglycerol O-acyltransferase 1, MGAT1) is encoded by the Moghat1 (also known as Dgat2l1) gene (Gene ID: 68393) in murine species. MGAT1 is an enzyme that catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Its activity is reported to be directly regulated by PPARg and it plays a role in regulating the pathway that leads to incorporation of fatty acids into triglycerides. MGAT1 activity is shown to be higher in mice fed high fat diet and by overexpression of PPARg, which leads to hepatic steatosis. Even in cultured hepatocytes, overexpression of MGAT1 is shown to increase triglyceride synthesis without affecting the expression of PPARg. The binding of PPARg to MGAT1 promoter is significantly enhanced Ob/Ob and high fat diet fed mice. MGAT1 expression is significantly reduced in PPARg knockdown murine models and knockdown of MGAT1 expression is shown to improve hepatic steatosis in high fat fed mice. It has also been shown that C3H mice that do not express PPARg and MGAT1 in the liver do not develop hepatic steatosis after being fed a high fat diet. (Ref.: Lee, YJ., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34); 13656-13661).

특이성

Clone 5F5 is a mouse monoclonal antibody that detects Monoacylglycerol O-acyltransferase 1 (MGAT1). It targets an epitope within 13 amino acids from the C-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of murine 2-acylglycerol O-acyltransferase 1 (MGAT1).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells overexpressing MGAT1.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MGAT1 in lysates from HEK293 cells transfected with mouse MGAT1, but not in untransfected cells.

Tested Applications

Western Blotting Analysis: A representative lot detected MGAT1 in Western Blotting applications (Lee, Y.J., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34):13656-61).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MGAT1, clone 5F5, Cat. No. MABS2219, is a mouse monoclonal antibody that detects 2-acylglycerol O-acyltransferase 1 (MGAT1) and is tested for use in Western Blotting,

물리적 형태

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable