MABS2217

Anti-Syntaxin 5 Antibody

purified antibody, clone 1G11, from mouse

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1G11, monoclonal
• 분자량: calculated mol wt 34 kDa; observed mol wt ~40 kDa
• 종 반응성: rat
• 종 반응성(상동성에 의해 예측): mouse, human, bovine
• 포장: antibody small pack of 100 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG
• 배송 상태: dry ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

특이성

Clone 1G11 is a mouse monoclonal antibody that detects Syntaxin-5. It targets an epitope within the cytoplasmic domain in the N-terminal half. It detects both isoform 1 and 2.

면역원

GST-tagged recombinant fragment corresponding to 83 amino acids from the C-terminal half, SNARE motif.

애플리케이션

Quality Control TestingEvaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Syntaxin-5 in Rat brain tissue lysate.Tested ApplicationsWestern Blotting Analysis: A representative lot detected Syntaxin-5 in Western Blotting applications (Williams, A.L., et. al. (2004). Mol Biol Cell. 15(1):162-75).Immunocytochemistry Analysis: A representative lot detected Syntaxin-5 in Immunocytochemistry Blotting applications (Williams, A.L., et. al. (2004). Mol Biol Cell. 15(1):162-75).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Syntaxin 5, clone 1G11, Cat. No. MABS2217, is a mouse monoclonal antibody that detects Syntaxin 5 and is tested for use in Immunocytochemistry and Western Blotting.

표적 설명

Syntaxin-5 (UniProt: Q08851; also known as STX-5) is encoded by the Stx5 (also known as Stx5a) gene (Gene ID: 65134) in rat. Syntaxin-5 is a single-pass type IV membrane protein that localizes throughout the Golgi apparatus and is most abundant in the cis-most cisternae. It is found in the endoplasmic reticulum (ER)-Golgi intermediate compartment membrane. It has a cytoplasmic domain (aa 1-333), a transmembrane domain (aa 334-354), and a single amino acid vesicular domain (aa 355). Its expression has been reported in the brain, heart, spleen, lung, liver, kidney, and testis. Syntaxin-5 mediates ER to Golgi transport and together with vesicular transport factor p115 (USO1) and Golgi matrix protein GM130 (GOLGA2), it is involved in tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Syntaxin-5 is shown to protect cells against proteotoxicity and is involved in clearance of insoluble protein deposits. (Ref.: Babazadeh, R., et al. (2019). Cell Rep. 28(8); 2096-2110; Diao, A., et al (2008); J. Biol. Chem. 283(11); 6957-6967).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at +2°C to +8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable