MABS2067

Anti-HSD3B2 Antibody, clone 6

clone 6, from mouse

• 동의어(들): 3-beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 6, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_001159592.1
• UniProt 수납 번호: P26439
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HSD3B2(3284)

설명

일반 설명

3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. (3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. 3-beta-HSD II is a single-pass membrane protein found on mitochondrial and endoplasmic reticulum membranes. It is expressed in adrenal gland, testis and ovary. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. Mutations in HSD3B2 gene are reported to cause adrenal hyperplasia that is characterized by androgen excess, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormone hypersecretion (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).

특이성

Clone 6 specifically detects HSDB32 in human tissue. it targets an epitope with in 14 amino acids from the N-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (3-beta-HSD).

애플리케이션

Anti-HSD3B2, clone 6, Cat. No. MABS2067, is a mouse monoclonal antibody that detects 3-beta-HSD II (HSD3B2) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B2 in Immunohistochemistry appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: Analysis: A representative lot detected HSD3B2 in Western Blotting appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected HSD3B2 in human adrenal gland and human placenta tissue sections.

ELISA Analysis: A representative lot detected HSD3B2 in ELISA appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Research Category
Signaling

품질

Evaluated by Western Blotting in human adrenal gland tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected HSD3B2 in human adrenal gland tissue lysate.

표적 설명

~40 kDa observed; 42.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.