MABS1201

Anti-OSBP Antibody, clone 1A6.2

clone 1A6.2, from mouse

• 동의어(들): Oxysterol-binding protein 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1A6.2, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_002547
• UniProt 수납 번호: P22059
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... OSBP(5007)

설명

일반 설명

Oxysterol-binding protein 1 (UniProt P22059) is encoded by the OSBP (also known as OSBP1) gene (Gene ID 5007) in human. OSBP mediates the transport of cholesterol and phosphatidylinositol 4-phosphate (PtdIns4P, PI-4-P, PI4P) between endoplasmic reticulum (ER) and Golgi. OSBP links ER and trans-Golgi membranes at the ER-Golgi membrane contact sites (MCSs) and shuttles sterol into the Golgi and PI4P back to the ER, where it is hydrolyzed by the PI4P-phosphatase Sac1. OSBP contains an FFAT (phenylalanines (FF) in an acidic tract) motif (a.a. 358-364) that binds ER-resident VAP protein and an N-terminal pleckstrin homology (PH) domain (a.a. 88-181) that binds PI4P. OSBP and OSBP-related protein 4 (ORP4) are reported to be involved in virus-induced membrane alterations essential for viral replication organelle formation. OSBP knockdown inhibits virus replication, whereas overexpression of OSBP or ORP4 counteracts the antiviral effects of the OSBP/ORP4 antagonist OSW-1.

특이성

Clone 1A6.2 targets an epitope in the C-terminal region of human OSBP.

면역원

Epitope: C-terminal region.

GST-tagged recombinant human OSBP C-terminal fragment.

애플리케이션

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSBP in human cerebral cortex, small intestine, and kidney tissue sections.

Research Category
Signaling

Research Sub Category
Vesicular Trafficking

This Anti-OSBP Antibody, clone 1A6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of OSBP.

품질

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected OSBP in 10 µg of Jurkat cell lysate.

표적 설명

~90 kDa observed. 89.42 kDa calculated. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1