MABS1164

Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1

clone 3B3.1, from mouse

• 동의어(들): Nucleobindin-2, Nestafin-1, DNA-binding protein NEFA, Prepronesfatin, Pronesfatin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3B3.1, monoclonal
• 종 반응성: human, mouse, rat
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_067695
• UniProt 수납 번호: Q9JI85
• 타겟 번역 후 변형: unmodified
• 유전자 정보: rat ... Nucb2(59295)

설명

일반 설명

Nucleobindin-2 (UniProt Q9JI85; also known as DNA-binding protein NEFA, Prepronesfatin) is encoded by the Nucb2 (also known as Nefa) gene (Gene ID 59295) in rat species. Nucleobindin-2 is initially translated as a 420-a.a. precursor protein. Cleavage of signal peptide (aa1-24) yields the 396-a.a. mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (more than 85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobindin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. Nucleobindin-2 is known to be expressed in key hypothalamic nuclei and plays a role in energy homeostasis. Nucleobindin-2 is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissues, suggesting roles in various metabolic functions. Nucleobindin-2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues.

특이성

Clone 3B3.1 reacts with pro-nucleobindin-2, mature nucleobindin-2, and nesfatin-1, but not nesfatin-2 or nesfatin-3.

면역원

Epitope: Nesfatin-1

GST-tagged recombinant rat Nestafin-1.

애플리케이션

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nucleobindin-2/nestafin-1 immunoreactivity in human colon cancer tissue, as well as in normal testis, tonsil, and pancreas tissue sections.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

This Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1.

품질

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate.

표적 설명

~50 kDa observed. 47.33/50.20 kDa (human mature/pro-nucleobindin-2), 47.42/50.30 kDa (mouse mature/pro-nucleobindin-2), 47.25/50.09 kDa (rat mature/pro-nucleobindin-2), 9.552/9.612/9.583 kDa (human/mouse/rat nesfatin-1) calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable