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Merck
모든 사진(2)

주요 문서

MABN894

Sigma-Aldrich

Anti-Synapsin-1 Antibody, clone 10.22

clone 10.22, from mouse

동의어(들):

Synapsin I, Synapsin-1

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

10.22, monoclonal

종 반응성

rat, mouse, human, fish, bovine

기술

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... SYN1(6853)

일반 설명

Synapsin-1 (UniProt P17599; also known as Synapsin I) is encoded by the SYN1 gene (Gene ID 281510) in bovine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided into A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The C-terminal region contains spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

특이성

Clone 10.22 reacts with both synapsin-1 spliced isoforms (Ia and Ib), but not synapsin-2 spliced isoform IIa or IIb (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).

면역원

Epitope: Pro-rich domain D.
Purified bovine brain synapsin-1.

애플리케이션

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synapsin-1 in human, mouse, and rat cerebral cortex tissues.
Western Blotting Analysis: A representative lot detected synapsin Ia/Ib in mouse cortical neuron lysates (Medrihan, L., et al. (2013). Nat. Commun. 4:1512).
Western Blotting Analysis: A representative lot detected purified bovine brain synapsin Ia/Ib (Messa, M., et al. (2010). J. Cell Sci. 123(13):2256-2265).
Western Blotting Analysis: A representative lot detected synapsin-1 in Torpedo (electric ray) synaptosomal preparations and in GST-cyclophilin B pull-downs (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411).
Western Blotting Analysis: A representative lot detected synapsin-1 in the same rat brain subcellular fractions as cyclophilin B (Lane-Guermonprez, L., et al. (2005). J. Neurochem. 93(6):1401-1411).
Western Blotting Analysis: A representative lot detected synapsin Ia/Ib, but not IIa/IIb, in rat brain post-nuclear supernatants (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin Ia/Ib, but not IIa/IIb, from rat brain synaptosomal preparations using protein G beads with pre-bound rabbit anti-mouse IgG (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunofluorescence Analysis: Clone 10.22 ascites fluid was employed to localize synapsin-1 immunoreactivity within retinal inner plexiform layer (IPL) of floating or whole-mount rat retinas (Mandell, J.W., et al. (1992). J. Neurosci. 12(5):1736-1749).
Note: Clone 10.22 does not bind significantly to protein G. For immunoprecipitation application, pre-coat protein G beads with an anti-mouse IgG antibody is recommended (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-Synapsin-1 Antibody, clone 10.22 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence for the detection of Synapsin-1.

품질

Evaluated by Western Blotting in rat brain cytosol tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-1 in 10 µg of rat brain cytosol tissue lysate.

표적 설명

~75 kDa observed.

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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