MABN717

Anti-WNT10B Antibody, clone 5A7

ascites fluid, clone 5A7, from mouse

• 동의어(들): Protein Wnt-10b, Protein Wnt-12
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 5A7, monoclonal
• 종 반응성: human
• 기술: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_003385
• UniProt 수납 번호: O00744
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... WNT10B(7480)

설명

일반 설명

Wingless-type MMTV integration site family member 10B (WNT10B), also known as Protein Wnt-12 (WNT12), consists of structurally related secreted signaling proteins implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is detected in most adult tissues, with the highest levels found in heart and skeletal muscle, and lower levels found in brain. WNT10B may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. Defects in WNT10B are linked to split-hand/foot malformation type 6 (SHFM6).

면역원

Purified recombinant fragment of human WNT10B expressed in E. coli.

애플리케이션

Anti-WNT10B Antibody, clone 5A7 is a highly specific mouse monoclonal antibody, that targets Wnt & has been tested in IHC, western blotting & Immunofluorescence.

Immunohistochemistry Analysis: A 1:500-1,000 dilution from a representative lot detected WNT10B in human kidney and liver tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT10B in PANC-1 cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WNT10B in 10 µg of HeLa cell lysate.

표적 설명

~47 kDa observed. Uncharacterized bands may appear in some lysate(s).

물리적 형태

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
HeLa cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable