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Merck
모든 사진(1)

Key Documents

MABN713

Sigma-Aldrich

Anti-CA9 Antibody, clone 2D3

ascites fluid, clone 2D3, from mouse

동의어(들):

Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Membrane antigen MN, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

2D3, monoclonal

종 반응성

human

기술

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG1

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... CA9(768)

일반 설명

CA9 (Carbonic anhydrase 9) is a single-pass type I membrane protein belonging to the alpha-carbonic anhydrase family. CA9 may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. CA9 is primarily expressed in carcinoma cell lines and is found on the surface microvilli and in the nucleus.

면역원

Purified recombinant fragment of human CA9 expressed in E. Coli.

애플리케이션

Anti-CA9 Antibody, clone 2D3 is a highly specific mouse monoclonal antibody, that targets Carbonic Anhydrase & has been tested in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CA9 in human stomach and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

품질

Evaluated by Western Blotting in HeLa and A549 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.

표적 설명

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

물리적 형태

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
HeLa and A549 cell lysates.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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