MABN627

Anti-SDHB Antibody, clone 10C5.1

clone 10C5.1, from mouse

• 동의어(들): Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDHB, Iron-sulfur subunit of complex II, Ip
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 10C5.1, monoclonal
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_002991
• UniProt 수납 번호: P21912
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SDHB(6390)

설명

일반 설명

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1; UniProt P21912; also known as Ip, Iron-sulfur subunit of complex II) is encoded by the SDHB (also known as IP, SDH, CWS2, PGL4, SDH1, SDHIP) gene (Gene ID 6390) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential cancer markers and targets for treatment. Succinate dehydrogenase (SDH), also known as mitochondrial respiratory Complex II, is a holoenzyme consisting of four essential subunits, SDHA (a flavoprotein), SDHB (iron-sulfur protein), and two membrane anchor units SDHC and SDHD. SDH assembly requires two factors, SDHAF1 and SDHAF2, while its function is influenced by the deacetylase activity of SIRT3. SDH is the only enzyme complex known to participate in both the TCA cycle and electron transport chain (ETC.). SDH catalyzes the oxidation of succinate in the TCA to form fumarate, a reaction that is coupled to the reduction of ubiquinone to ubiquinol in the ETC. SDH loss-of-function mutations have been linked to pheochromocytoma (PCC), paraganglioma (PGL), gastrointestinal stromal tumor (GIST), renal cell carcinoma, and ovarian cancer.

면역원

Epitope: C-terminal half

GST-tagged recombinant protein corresponding to the C-terminal half of human SDHB.

애플리케이션

Anti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SDHB in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.

품질

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.

표적 설명

~30 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable