MABN543

Anti-Gs protein Antibody, alpha subunit, clone N192/12

clone N192/12, from mouse

• 동의어(들): Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: N192/12, monoclonal
• 종 반응성: rat, human
• 종 반응성(상동성에 의해 예측): bovine (immunogen homology)
• 기술: western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_851364
• UniProt 수납 번호: P04896
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GNAS(2778)

설명

일반 설명

The heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) mediate signaling from a large number of G-protein coupled receptors (GPCR) to a variety of intracellular effectors. G-proteins are composed of a 36-52 kDa α-subunit, a 35-36 kDa β-subunit, and an 8-10 kDa γ-subunit. They are classified based on the nature of their α-subunit. Gs is ubiquitously expressed and couples receptors to adenylyl cyclase and promotes intracellular cAMP generation. Mutations in Gs-α can lead to constitutive activation of receptors leading to development of endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. In brain tissue Gs-α activates adenyl cyclase in direct response to stimulation of several neurotransmitter receptors and facilitates depolarization-induced neurotransmitter release for synaptic transmission. Also, it is shown that Gs-stimulated adenylyl cyclase activity is significantly reduced in the Alzheimer′s disease brain tissue.

특이성

Other homologies: Mouse (99% sequence homology).

면역원

Recombinant protein corresponding to bovine GS protein, alpha subunit.

애플리케이션

Anti-Gs protein Antibody, alpha subunit, clone N192/12 is a highly specific mouse monoclonal antibody & that targets Gsα & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GS protein, alpha subunit in 10 µg of SH-SY5Y cell lysate.

품질

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GS protein, alpha subunit in 10 µg of rat brain membrane tissue lysate.

표적 설명

~45 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat brain membrane tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable