MABN2727

Anti-Pan Lhx1/5 Antibody, clone 4F2

• 동의어(들): LIM/homeobox protein Lhx5
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4F2, monoclonal
• 분자량: calculated mol wt 45 kDa; observed mol wt ~48 kDa
• 정제법: using protein G
• 종 반응성: mouse, rat, human, chicken
• 포장: antibody small pack of 100
• 기술: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_620605
• UniProt 수납 번호: P61376
• 저장 온도: 2-8°C
• 유전자 정보: rat ... Lhx1(124451)

설명

특이성

Clone 4F2 is a mouse monoclonal antibody that detects.

면역원

Recombinant rat Lim2/LhxV5 protein.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Lhx1/5 in Mouse brain tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Lhx1/5 in Immunocytochemistry applications (Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316).

Immunohistochemistry Applications: A representative lot detected Pan Lhx1/5 in Immunohistochemistry applications (Lu, Y., et al. (2020). Dev Cell. 53(4):473_491.e9; Shirazi Fard, S., et al. (2014). Cell Cycle. 13(3):408-17; Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).

Immunofluorescence Analysis: A representative lot detected Pan Lhx1/5 in Immunofluorescence applications (Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40; Samson, A., et al. (2018). Gut. 67(3):562-573).

Western Blotting Analysis: A representative lot detected Pan Lhx1/5 in Western Blotting applications (Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

LIM/homeobox protein Lhx5 (UniProt: P61376; also known as LIM homeobox protein 5, Homeobox protein LIM-2) is encoded by the Lhx5 (also known as Lim-2, Lim2) gene (Gene ID: 124451) in rat. Lhx5 is a nuclear protein that belongs to a family of proteins that carry the LIM domain. Its LIM zinc-binding domains are localized in amino acids 3-61 and 62-125. It acts as a transcriptional regulator and is reported to play an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system. Its expression is detected in developing Purkinje cells soon after they exited the cell cycle and migrate out of the cerebellar ventricular zone. Lhx5 is also required for the generation of the mamillary body (MM) and its derived tracts that are related to memory and spatial navigation that interconnect hippocampal, thalamic, and tegmental regions. It is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Lhx5 is reported to regulate the local inhibition of Wnt proteins. Activation of Lhx5 is shown to expand forebrain structures. It can rescue forebrain deficiencies caused by excess Wnt activity. Its inhibition results in ectopic activation of Wnt signaling. It regulates the expression of two secreted Frizzled-related Wnt antagonists, Sfrp1a and Sfrp5 that can reduce the ectopic activation of Wnt signaling and rescue the forebrain deficiencies caused by inhibition of Lhx5 function. (Ref.: Miquelajáuregui, A., et al. (2015) Front. Neuroanat. 9; 136; Yang, M., et al. (2010). J. Comp. Neurol. 518(14); 2818-2840; Peng, G., and Westerfield, M. (2006). Development. 133(16); 3191-3200).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable