추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies, primary antibodies
클론
C65-2, monoclonal
분자량
calculated mol wt 27.54 kDa
observed mol wt ~29 kDa
정제법
using protein G
종 반응성
human, monkey
포장
antibody small pack of 100 μg
기술
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgMκ
에피토프 서열
C-terminal
단백질 ID 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... FTMT(94033)
일반 설명
Ferritin, mitochondrial (UniProt: Q8N4E7; also known as EC:1.16.3.1, FTMT) is encoded by the FTMT gene (Gene ID: 94033) in human. Mitochondria are shown to be the sites where iron is transformed into heme and Fe-S-clusters. Mitochondrial ferritin (FtMt), a novel H-type ferritin, with ferroxidase activity. It is a homooligomeric protein with 24 subunits that is synthesized with a transit peptide (aa 1-49) that is subsequently cleaved off in the mitochondrial matrix to produce the mature protein (aa 50-242). The functional molecule is roughly spherical and contains a central cavity into which the polymeric mineral iron core is deposited. It stores iron in a soluble, non-toxic, readily available form. It is detected in testis and erythroleukemia and is also expressed in very low levels in the brain and other tissues. FtMt levels appear to correlate more with mitochondrial abundance than with iron metabolism. Higher levels of FtMt are reported in the cerebral cortex of Alzheimer s disease patients and in the substantia nigra of subjects with Parkinson s disease. Although FtMt exhibits about 79% homology to H-chain ferritin (FTH), Clone C65-2 specifically recognize only the FtMt and does not react with FTH. (Ref.: Yang, M., et al. (2017). Acta Histochem. Cytochem. 50(1); 49-55; Yang, H., et al. (2013). Neurosci. Res. 77(1-2); 1-7).
특이성
Clone C65-2 is a mouse monoclonal antibody that detects mitochondrial ferritin. It targets an epitope within nine amino acids from the C-terminal region.
면역원
KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human mitochondrial ferritin.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in lysates from HEK293 cells overexpressing Ferritin mitochondrial (FTMT).
Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Ferritin, mitochondrial (FTMT) in lysate from HEK293 cells overexpressing FTMT.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Mitochondrial ferritin/FTMT in Immunohistochemistry applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Western Blotting Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Western Blotting applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Immunofluorescence Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Immunofluorescence applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysates from HEK293 cells overexpressing Ferritin mitochondrial (FTMT).
Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Ferritin, mitochondrial (FTMT) in lysate from HEK293 cells overexpressing FTMT.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Mitochondrial ferritin/FTMT in Immunohistochemistry applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Western Blotting Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Western Blotting applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Immunofluorescence Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Immunofluorescence applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mitochondrial ferritin/FTMT clone C65-2 , Cat. No. MABN2635, is a mouse monoclonal antibody that detects mitochondrial ferritin and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
물리적 형태
Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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