MABN2592

Anti-RBP3 Antibody, clone mAb5

• 동의어(들): IRBP, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, Protein 7S, Retinol-binding protein 3
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: mAb5, monoclonal
• 종 반응성: bovine
• 포장: antibody small pack of 100
• 기술: electron microscopy: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: N-terminal half
• 단백질 ID 수납 번호: NP_776589
• UniProt 수납 번호: P12661
• 유전자 정보: bovine ... RBP3(281443)

설명

특이성

Clone mAb5 is a mouse monoclonal antibody that detects Retinol-binding protein 3 (RBP3). It targets an epitope within the N-terminal half.

면역원

Purified Retinol-binding protein 3 (RBP3) from bovine retina.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from Bovine retinal tissue.

Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.

Tested Applications

Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Retinol-binding protein 3 (UniProt: P12661; also known as Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3, Protein 7S) is encoded by the RBP3 gene (Gene ID: 281443) in bovine. RBP3 is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-22) that are subsequently cleaved off to produce the mature form. It is a lipophilic glycoprotein that is secreted by rod and cone photoreceptors and plays a major role in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Its expression is observed early in retinal differentiation. RBP3 shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. It can bind many retinoids and polyunsaturated fatty acids and in addition to retinoid binding, it also plays a role in retinal development and homeostasis. RBP3 also displays thiol-dependent antioxidant activity that maintains the delicate redox balance in the normal retina. RBP3 dysregulation has been linked to retinal diseases. Mice lacking RBP3 exhibit severe early and progressive photoreceptor degeneration that is characterized by a reduction in both length and numbers of cone sheaths. In mammals four protein subunits of RBP3 have been described (M1-4), each consisting of about 300 amino acids. Clone mAb5 has been mapped to M2 subunit of RBP3. (Ref.: Sears, AE., et al. (2020) FASEB J. 34(10); 13918-13934; Zheng, S., et al. (2020). Front. Cell. Neurosci. 14; Article 577935).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable