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Merck
모든 사진(6)

Key Documents

MABN1832

Sigma-Aldrich

Anti-SK2 (KCa2.2) Antibody, clone K78/29

clone K78/29, from mouse

동의어(들):

Small conductance calcium-activated potassium channel protein 2, SK2, SKCa 2, SKCa2, KCa2.2

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

K78/29, monoclonal

종 반응성

human, rat, mouse

기술

immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... KCNN2(3781)

일반 설명

Small conductance calcium-activated potassium channel protein 2 (UniProt P70604; also known as SK2, SKCa 2, SKCa2, KCa2.2) is encoded by the Kcnn2 gene (Gene ID 54262) in rat species. Small conductance calcium-activated potassium channels mediate cell membrane repolarization following intracellular Ca2+ increase induced by various stimuli, thereby restoring an excited cell back to its resting state. SK2 is involved in afterhyperpolarization (AHP) that follows action potentials in vertebrate neurons. SK2 regulates neuronal excitability by contributing to the slow component of synaptic AHP. Ca2+ activates SK2 through the Ca2+ sensor calmodulin (CaM), which can bind up to four Ca2+ ions. The dynamic coupling between Ca2+ binding to CaM, CaM binding to SK, and the open probability of SK are all essential components of the SK2 activity.

특이성

Clone K78/29 detects endogenous KCa2.2 in rat brain membrane extracts, as well as the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells. Exprected to react with both spliced isoforms of human KCa2.2 reported by UniProt (Q9H2S1).

면역원

Epitope: C-terminal cytomplasmic region.
Linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat SK2 (KCa2.2).

애플리케이션

Anti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected SK2 (KCa2.2) immunoreactivity in human cerebellum, human cerebral cortex, mouse brain, mouse skeletal muscle, rat kidney and rat testis tissue sections.
Western Blotting Analysis: A representative lot detected the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Immunohistochemistry Analysis: A representative lot detected SK2 (KCa2.2) immunoreactivity in adult rat brain hippocampus sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

품질

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SK2 (KCa2.2) in 10 µg rat brain tissue lysate.

표적 설명

~45 kDa observed. 63.85 kDa (rat), 63.76 kDa (human isoform 1), 26.34 kDa (human isoform 2), 91.60 kDa (mouse) calculated.

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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