MABN1595

Anti-Serotonin receptor 2A/HTR2A Antibody, clone 9B11.1

clone 9B11.1, from mouse

• 동의어(들): 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 9B11.1, monoclonal
• 종 반응성: mouse, rat, human
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_000612
• UniProt 수납 번호: P28223
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HTR2A(3356)

설명

일반 설명

5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor. Human 5-HT2A receptor contains four extracellular domains (a.a. 1-75, 133-148, 216-233, 347-362), seven transmembrane segments (a.a. 76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a. 100-110, 172-191, 255-324, 385-471).

특이성

Expected to react with both spliced isoforms of human Serotonin receptor 2A reported by UniProt (P28223).

면역원

Epitope: Third cytoplasmic domain.

GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.

애플리케이션

Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected 2A/HTR2A in human cerebral cortex, mouse cerebral cortex, and rat testis tissue sections.

Research Category
Neuroscience

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Serotonin receptor 2A/HTR2A Antibody, clone 9B11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of SHTR2A.

품질

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 2A/HTR2A in 10 µg of human cerebral cortex tissue lysate.

표적 설명

~52 kDa observed. 52.60 kDa (isoform 1) and 43.94 kDa (isoform 2) calculated.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable