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Merck
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Key Documents

MABF836

Sigma-Aldrich

Anti-EBI3 Antibody/IL-35, clone V1.4H6.25 (Neutralizing)

clone V1.4H6.25, from mouse

동의어(들):

EBI3/IL-35, IL-27B, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein homolog

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

V1.4H6.25, monoclonal

종 반응성

mouse

기술

neutralization: suitable
western blot: suitable

동형

IgG3κ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Ebi3(50498)

일반 설명

EBI3/IL27B is the beta subunit of the functional cytokine IL-27. The beta subunit is required for activity. IL-27 is a critical cytokine of mammalian innate immune response. IL-27 has both pro and anti-inflammatory activities. IL-27 regulates T-helper cell development and differentiation into TH1, TH2 and TH17 effector cells. IL-27 also is important in isotype switching for B-cells, and it drives rapid clonal expansion of native but not memory CD4 cells. IL-27 is also important for its anti-tumor and anti-angiogenic activity on tumor and cancer cells. The beta subunit EBI3/IL27B is also a component of IL-35 which is a heterodimer of IL-12alpha and IL-27B/EBI3. IL-35 plays a role in immune suppression. IL-27 is a secreted cytokine, thus IL-27beta subunit is expressed intracellularly associated with the endoplasmic reticulum and membrane vesicles and is detected extracellularly as part of the mature cytokine as well.

특이성

Clone V1.4H6.25 (MABF836) was used in in-vitro mIL-35 neutralizing studies and clone V1.4C4.22 (MABF848) was used in in-vivo mIL-35 neutralizing studies (Collison, L.W., et al. (2010). Nat Immunol. 11(12):1093-1101).

면역원

Recombinant protein corresponding to mouse EBI3/IL-35.

애플리케이션

Anti-EBI3 Antibody/IL-35, clone V1.4H6.25 (Neutralizing) is an antibody against EBI3 /IL-35 for use in Western Blotting, Neutralizing.
Neutralizing Analysis: A representative lot detected EBI3/IL-35 during in-vitro neutralizing (Collison, L.W., et al. (2010). Nat Immunol. 11(12):1093-1101).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

품질

Evaluated by Western Blotting in recombinant mouse EBI3 protein.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected EBI3/IL-35 in 1 µg of recombinant mouse EBI3 protein.

표적 설명

~25 kDa observed

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG3κ in buffer containing PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Nature immunology
Collison, LW; Chaturvedi, V; Henderson, AL; Giacomin, PR; Guy, C; Bankoti, J; Finkelstein et al.
Nature Immunology null

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