MABF3208

Anti-HPV11 Antibody, clone H11.H3

• 동의어(들): HPV-11, Major capsid protein L1
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: H11.H3, monoclonal
• 분자량: calculated mol wt 55.8 kDa; observed mol wt ~N/A kDa
• 정제법: using protein G
• 종 반응성: virus
• 포장: antibody small pack of 100
• 기술: ELISA: suitable; neutralization: suitable
• 동형: IgG2bκ
• 단백질 ID 수납 번호: N/A
• UniProt 수납 번호: P04012
• 저장 온도: -10 to -25°C

설명

특이성

Clone H11.H3 is a mouse monoclonal antibody that detects HPV11. It targets an epitope within the Major capsid protein L1.

면역원

Intact HPV-11 virions obtained from morphologically transformed human foreskin xenografts grown subrenally in athymic mice.

애플리케이션

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b, kappa.

Tested Applications

Enzyme Immunoassay Analysis: A representative lot of this antibody detected HPV11 protein in ELISA application.

Neutralizing: A representative lot of this antibody neutralized HPV11 in neutralizing application (Christensen, N.D., et al. (1994). Virology. 205(1):329-35; Christensen, N.D., et al. (1996). Virology. 224(2):477-86).

ELISA Analysis: A representative lot of this antibody detected HPV11 protein in ELISA application (Christensen, N.D., et al. (1990). J Virol. 64(11):5678-81; Christensen, N.D., et al. (1994). Virology. 205(1):329-35 ; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Panicker, G., et al. (2021). J Immunol Methods. 498:113136; Jerajani, K., et al. (2022). J Pharm Sci. 111(11):2983-2997).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Major capsid protein L1 (UniProt: P04012) is encoded by the L1 gene in Human papillomavirus 11. The Major Capsid Protein L1 of Human papillomavirus is the primary structural protein responsible for the formation of the viral capsid, that encapsulates the genomic DNA together with Minor Capsid Protein L2. The viral capsid is composed of 360 copies of the L1 protein forming 72 pentamers, that assemble into the icosahedral capsid structure, while the L2 protein localizes along the inner surface of the virion and plays a role in capsid stabilization through interaction with the Major Capsid Protein L1. The L1 protein facilitates the attachment of the virus to the host cell by interacting with heparan sulfate proteoglycans on the cell surface, a critical step for infection. Recombinantly expressed Major Capsid Protein L1 can spontaneously assemble into Virus Like-Particles (VLPs) and were used for the development of neutralizing antibodies that show high degree of Human papillomavirus type-specificity. Based on their oncogenic potential, Human papillomaviruses (HPVs) can be classified as low-risk HPV types, (e.g.: HPV6 and HPV11, the most common serotypes) which do not cause cancer but can lead to skin and genital warts, and high-risk HPV types (e.g.: HPV 16, 18, 31, 33, 35, 45, 52, 58), that are responsible for HPV related cancers. Vaccines targeting the L1 protein, have been developed and are effective in preventing infection with the most oncogenic HPV types by inducing a robust antibody response. (Ref.: Hareza, D. A., (2022) Int. J. Mol. Sci., 23, 1818.; Christensen, N.D., et al. (1990). J Virol. 64:11, 5678-81; Christensen, N.D., et al. (1996). Virology. 224(2):477-86; Brown, M.J., et al. (2014). Clin Vaccine Immunol. 21:4, 587-93)

물리적 형태

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable