MABF3182

Anti-Flt3L Antibody, clone 11C9.10.1

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 11C9.10.1, monoclonal
• 분자량: calculated mol wt 26.42 kDa; observed mol wt ~27 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Extracellular domain
• 단백질 ID 수납 번호: NP_001450.2
• UniProt 수납 번호: P49771
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FLT3LG(2323)

설명

일반 설명

Fms-related tyrosine kinase 3 ligand (UniProt: P49771; also known as Flt3 ligand, Flt3L, SL cytokine) is encoded by the FLT3LG gene (Gene ID: 2323) in human. Flt3 ligand is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 27-184), a transmembrane domain (aa 185-205), and a cytoplasmic domain (aa 206-235). Flt3-ligand is an important regulator of hematopoiesis that synergizes with a number of colony-stimulating factors and interleukins. It is expressed on myeloid, lymphoid, and dendritic cell progenitors. Expression of its receptor is restricted only to hematopoietic progenitors, early B cells and myeloid cells. Binding of Flt3-ligand to its receptor (Flt3) leads to receptor homodimerization and subsequent conformational changes that result in phosphorylation of its tyrosine kinase domains. This leads to rapid activation of receptor, its internalization, downstream actions, and degradation. Administration of Flt-3 ligand to mice is shown to cause a significant expansion of both CD8 + and CD8 dendritic cells at multiple sites, including spleen, lymph nodes, thymus, liver, lung, and bone marrow. Three isoforms of Flt3 have been described that are produced by alternative splicing. Isoform one is a single-pass type I membrane protein, isoform 2 is a homodimeric secreted or extracellular protein, and isoform 3 lacks the first 82 amino acids. (Ref.: Tsapogas, P., et al. (2017). Int. J. Mol. Sci. 18(6): 1115).

특이성

Clone 11C9.10.1 is a mouse monoclonal antibody that detects Fms-related tyrosine kinase 3 ligand (Flt3L). It targets an epitope within the extracellular domain.

면역원

His-tagged recombinant fragment corresponding to the 158 amino acids from the N-terminal, extracellular domain of human Fms-related tyrosine kinase 3 ligand (Flt3L).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting with recombinant Flt3L protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant Flt3L.

Tested Applications

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Flt3L in HeLa and A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Flt3L, clone 11C9.10.1, Cat. No. MABF3182, is mouse monoclonal antibody that detects Flt3L and is tested for use in Immunocytochemistry and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2