MABF2781

Anti-LCMV Nucleoprotein Antibody, clone VL-4

• 동의어(들): LCMV Nucleocapsid Protein, Protein N
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: VL-4, monoclonal
• 정제법: using protein G
• 종 반응성: virus
• 포장: antibody small pack of 100 μL
• 기술: ELISA: suitable; activity assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; neutralization: suitable
• 동형: IgG2aκ
• UniProt 수납 번호: P07400
• 저장 온도: -10 to -25°C
• 유전자 정보: vaccinia virus ... N(956592)

설명

특이성

Clone VL-4 is a rat monoclonal antibody that detects nucleoprotein of Lymphocytic choriomeningitis virus (LCMV).

면역원

Lymphocytic choriomeningitis virus (LCMV).

애플리케이션

Quality Control Testing

Evaluated by ELISA with recombinant LCMV nucleoprotein .

ELISA Analysis: A serial dilution of this antibody detected recombinant CLMV nucleoprotein.

Tested Applications

ELISA Analysis: A representative lot detected LCMV Nucleoprotein in ELISA application (Eschli, B., et al. (2007). J Virol. 81(21):11650-7).

Flow Cytometry Analysis: A representative lot detected LCMV Nucleoprotein in Flow Cytometry application (Herz, J., et al. (2015). J Exp Med. 212(8):1153-69).

Neutralizing: A representative lot detected LCMV Nucleoprotein in Neutralizing applications (Eschli, B., et al. (2007). J Virol. 81(21):11650-7).

Activity Assay: A representative lot detected Nucleoprotein in lymphocytic choriomeningitis virus (LCMV) (Data courtesy of the Division of Experimental Virology, Department of Biomedicine, University of Basel, Switzerland).

Immunohistochemistry Applications: A representative lot detected LCMV Nucleoprotein in Immunohistochemistry applications (Herz, J., et al. (2015). J Exp Med. 212(8):1153-69; Urata, S., et al. (2018). PLoS Pathog. 14(7):e1007172).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Nucleoprotein (UniProt: P07400; also known as Nucleocapsid protein, Protein N) is encoded by the N gene (Gene ID: 127897) in Lymphocytic choriomeningitis virus (LCMV). LCMV is a murine enveloped RNA virus of the arenavirus family. Its viral surface is densely covered with viral fusion glycoprotein spikes that form heterodimeric trimers, consisting of the peripheral receptor binding glycoprotein 1 (GP-1) and a transmembrane subunit (GP-2). The large segment of LCMV genome encodes for the RNA-dependent-RNA-polymerase (L) and the matrix protein (Z) that mediates viral assembly and budding. Its small segment (S) encodes the glycoprotein precursor (GPC) and the viral nucleoprotein (NP). GPC is then co-translationally cleaved by signal peptidase to produce a stable 58 amino acid stable signal peptide (SSP) and GPC that is post-translationally processed by the cellular site 1 protease to form GP-1 and GP-2. GP-1 is reported to mediate virion attachment to the cell surface followed by cell entry via receptor-mediated endocytosis. The GP-2 subunit is responsible for the pH-dependent fusion event in the acidic environment of the endosome to complete the virus cell entry process and release of virus ribonucleoprotein into the cell cytoplasm to initiate transcription and replication of the viral genome. The nucleoprotein of LCMV encapsidates the genome and protects it from nucleases. The encapsidated genomic RNA known as the nucleocapsid (NC) serves as template for viral transcription and replication. Clone VL-4 specifically reacts with LCMV nucleoprotein and does not exhibit reactivity with vaccinia or influenza virus infected cells. (Ref.: Urata, S., et al. (2018). PLoS Pathog 14(7): e1007172; Eschli, B., et al. (2007). J. Virol. 81(21); 11650-11657).

물리적 형태

Purified rat monoclonal antibody IgG2a in PBS without azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable