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Merck
모든 사진(1)

주요 문서

MABF2771

Sigma-Aldrich

Anti-564/idiotype Antibody, clone 9D11

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

9D11, monoclonal

분자량

observed mol wt ~54 kDa

정제법

using protein G

종 반응성

mouse

포장

antibody small pack of 100 μL

동형

IgG1κ

에피토프 서열

Unknown

단백질 ID 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

일반 설명

An idiotype is the genetically determined variation of structures in the variable regions of immunoglobulins. Anti-idiotypic antibodies are known to target specific epitopes, on another antibody that are unique for that target antibody. The collection of epitopes for a given antibody defines its idiotype and is generally found in the complementarity determining regions of that targeted antibody. These antibodies can also bind shared epitopes on highly related monoclonal antibodies. Their specificity allows for the specific detection of antibody of interest even in the presence of endogenous polyclonal antibody. Due to their specificity for the variable region, these antibodies can also be useful in pharmacokinetic and immunogenicity studies. 564Igi is shown to be a reliable murine model of systemic lupus erythematous (SLE). These mice develop SLE profile closely resembling that of human SLE. These mice possess rearranged immunoglobulin heavy- and light-chain genes inserted into their respective loci that are together specific for nucleolar antigens. The 564 antibody is reported to have a characteristic idiotype and B cells carrying the corresponding B-cell antigen receptor are idiotype positive. The epitope recognized by the 564 idiotype antibody is a domain that may be common to multiple self-antigens. (Ref.: Berland, R., et al. (2006). Immunity 25(3); 429-440).

특이성

Clone 9D11 is a mouse monoclonal antibody that detects idiotype of a mouse IgG.

면역원

An idiotype of a mouse IgG (clone C11) representing B cell receptor expressed by auto reactive B cells in 564 Igi mice.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Idiotype of a mouse IgG target antigen (clone C11).

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected idiotype of a mouse IgG target antigen (clone C11) .

Tested Applications

Flow Cytometry Analysis: A representative lot detected 564/idiotype in Flow Cytometry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450; van der Poel, C.E., et al. (2019). Cell Rep. 29(9):2745-2755.e4; Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Immunohistochemistry Applications: A representative lot detected 564/idiotype in Immunohistochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunoprecipitation Analysis: A representative lot detected 564/idiotype in Immunoprecipitation applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunocytochemistry Analysis: A representative lot detected 564/idiotype in Immunocytochemistry applications (Chatterjee, P., et al. (2013). Eur J Immunol. 43(9):2441-2450).

Immunofluorescence Analysis: A representative lot detected 564/idiotype in Immunofluorescence applications (Simoni, L., et al. (2020). Cell Rep. 33(5):108330).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-564/idiotype, clone 9D11, Cat. No. MABF2771, is a mouse monoclonal antibody that detects mouse IgG idiotype and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, , Immunoprecipitation, & Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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