MABF2155

Anti-Ricin A Chain Antibody, clone RAC18

clone RAC18, from mouse

• 동의어(들): RAC
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: RAC18, monoclonal
• 종 반응성: Ricinus communis
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; inhibition assay: suitable; neutralization: suitable
• 동형: IgG2aκ
• UniProt 수납 번호: Q0Q2G6
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Ricin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), whcih has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. This leads to inhibition of protein synthesis in cells. The toxic effects of Ricin are attributed to the A chain. Clone RAC18 displays high avidity for intact ricin and inhibits its activity without affecting binding of ricin to cells. Immunization with RAC18 is shown to offer significant protection against ricin toxicity. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228).

특이성

Clone RAC18 bind to Ricin A chain

면역원

Purified Ricin A chain.

애플리케이션

Anti-Ricin A Chain, clone RAC18, Cat. No. MABF2155, is a mouse monoclonal antibody that detects Ricin A chain and has been tested for use in ELISA, Inhibition Assay, and Neutralizing.

Neutralizing Analysis: A representative lot neutralized the activity of Ricin A chain. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin A chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).

Inhibition Analysis: A representative lot inhibited the enzymatic activity of Ricin. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

Research Category
Inflammation & Immunology

품질

Evaluated by ELISA with Ricin A chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin A chain in an indirect ELISA.

표적 설명

29.89 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.