MABF2073

Anti-Rabies Virus CVS-11 Antibody, clone 509-6

clone 509-6, from mouse

• 동의어(들): Glycoprotein, Glycoprotein Rabies virus, Rabies virus strain CVS-11
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 509-6, monoclonal
• 종 반응성: rabies virus
• 종 반응성(상동성에 의해 예측): all
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; flow cytometry: suitable; neutralization: suitable
• 동형: IgG2aκ
• UniProt 수납 번호: O92284
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Glycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672 4678).

특이성

Clone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells.

면역원

Beta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV).

애플리케이션

Anti-Rabies Virus, clone 509-6, Cat. No. MABF2073, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus, strain CVS-11 and has been tested for use in ELISA, Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), and Neutralizing.

Neutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

ELISA Analysis: A representative lot detected Rabies Virus in ELISA applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Rabies Virus in Fluorescence Activated Cell Sorting (FACS) applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

Research Category
Inflammation & Immunology

품질

Evaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells.

Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein.

표적 설명

58.86 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable