MABE968

Anti-HLCS Antibody, clone 2C2.1

clone 2C2.1, from mouse

• 동의어(들): Biotin--protein ligase, HLCS, Biotin apo-protein ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase, Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, Holocarboxylase synthetase, HCS, Biotin--[methylcrotonoyl-CoA-carboxylase] ligas
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 2C2.1, monoclonal
• 종 반응성: human
• 기술: western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_000402
• UniProt 수납 번호: P50747
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HLCS(3141)

설명

일반 설명

Biotin-protein ligase (EC 6.3.4.9; EC 6.3.4.10; EC 6.3.4.11; EC 6.3.4.15; UniProt P50747; also known as Biotin-[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase, Biotin-[methylcrotonoyl-CoA-carboxylase] ligase, Biotin-[methylmalonyl-CoA-carboxytransferase] ligase, Biotin-[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, HCS, Holocarboxylase synthetase) is encoded by the HLCS (also known as HCS) gene (Gene ID 3141) in human. HLCS (holocarboxylase synthetase) is the sole biotin protein ligase encoded by the human genome. HLCS catalyses the covalent modification of target lysine residues by biotin. In addiition to taregting various carboxylases in the cytoplasm and mitochondria, both the full-length HLCS and a splicing variant are reported to enter the nucleus and associate with multi-protein gene repression complex. HLCS overexpression in HEK293 cells is reported to downregulate histone H3 lysine9 acetylation (H3K9ac) level with a cocomitant drop in LTRs (long terminal repeats) 15 and 22 due to a decrease in mRNA coding for LTRs. HLCS is also reported to induce RANTES expression in HEK-293 cells by biotinylating heat shock protein 72 (HSP72).

면역원

Epitope: N-terminal region

GST-tagged recombinant protein corresponding to the N-terminal region of human HLCS.

애플리케이션

Anti-HLCS Antibody, clone 2C2.1 is an antibody against HLCS for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

품질

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected HLCS in 10 µg of human skeletal muscle tissue lysate.

표적 설명

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable