MABE939

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody

clone 6G8B7, 1 mg/mL, from rat

• 동의어(들): H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: 6G8B7, monoclonal
• application: ELISA; ICC; WB
• 종 반응성: human
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 4

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 6G8B7, monoclonal
• 종 반응성: human
• 농도: 1 mg/mL
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_002098
• UniProt 수납 번호: P84243
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer10)
• 유전자 정보: human ... H3C1(8350)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

면역원

KLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).

애플리케이션

Immunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 (Ser10) in 10 µg of nocodazole treated HeLa cell lysates.

표적 설명

~17 kDa observed

결합

Replaces: 04-1093

물리적 형태

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable