추천 제품
생물학적 소스
Escherichia coli
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
BG4, monoclonal
종 반응성
human
포장
antibody small pack of 25 μL
기술
ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable
배송 상태
ambient
타겟 번역 후 변형
unmodified
일반 설명
DNA G-quadruplex structures are associated with a number of important aspects of genome function, which include transcription, recombination and replication. Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4), which can influence methylation at CpG islands (CGIs). G4 structure is reported to be tightly associated with CGI hypomethylation in the human genome. Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an attractive topic in molecular biomedical research.(Ref.: Mao, SQ et al., (2018). Nat. Struc. Mol. Biol. 25(10); 951-957).
특이성
Clone BG4 has a high affinity for intramolecular and intermolecular DNA G-quadruplexes with no detectable binding to a RNA hairpin, single-stranded DNA or double-stranded DNA.
면역원
G-quadruplex oligonucleotides.
애플리케이션
Anti-DNA G-quadruplex structures, clone BG4 Antibody, Cat. No. MABE917, is a highly specific monoclonal antibody that targets DNA G-quadruplex structures and has been tested in and ELISA, ChIP-seq, and Immunocytochemistry.
ELISA Analysis: A representative lot detected DNA G-quadruplex structures in Anti-DNA G-quarduplex structures, BG4 (Courtesy of University of Cambridge).
ChIP-seq Analysis: A representative lot detected DNA G-quadruplex structures in ChIP-seq applications (Hansel-Hertsch, R., et. al. (2016). Nat Genet. 48(10):1267-72).
ELISA Analysis: A representative lot detected DNA G-quadruplex structures in ELISA applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).
Immunocytochemistry Analysis: A representative lot detected DNA G-quadruplex structures in Immunocytochemistry applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).
ChIP-seq Analysis: A representative lot detected DNA G-quadruplex structures in ChIP-seq applications (Hansel-Hertsch, R., et. al. (2016). Nat Genet. 48(10):1267-72).
ELISA Analysis: A representative lot detected DNA G-quadruplex structures in ELISA applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).
Immunocytochemistry Analysis: A representative lot detected DNA G-quadruplex structures in Immunocytochemistry applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).
품질
The Anti-DNA-G-quadruplex structures, clone BG4 antibody (Cat. No. MABE917) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with Anti-FLAG epitope tag antibody (MAB3118).
물리적 형태
Format: Purified
Purified monoclonal antibody in PBS with 0.05% sodium azide.
기타 정보
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Lijun Xiang et al.
Nucleic acids research, 50(12), 6953-6967 (2022-06-25)
G-quadruplex structure (G4) is a type of DNA secondary structure that widely exists in the genomes of many organisms. G4s are believed to participate in multiple biological processes. Acyl-CoA binding protein (ACBP), a ubiquitously expressed and highly conserved protein in
Katharina Schlag et al.
The FEBS journal, 287(20), 4481-4499 (2020-02-26)
5-Lipoxygenase (5-LO) is the initial enzyme in the biosynthesis of leukotrienes, which are mediators involved in pathophysiological conditions such as asthma and certain cancer types. Knowledge of proteins involved in 5-LO pathway regulation, including gene regulatory proteins, is needed to
Zi-Fu Wang et al.
Cell chemical biology, 26(2), 179-190 (2018-12-06)
The most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) is an expanded G4C2 repeat [(G4C2)exp] in C9ORF72. ALS/FTD-associated toxicity has been traced to the RNA transcribed from the repeat expansion [r(G4C2)exp], which sequesters RNA-binding proteins
Xiaolin Wang et al.
iScience, 25(11), 105312-105312 (2022-10-29)
DNA G-quadruplex is a non-canonical secondary structure that could epigenetically regulate gene expression. To investigate the regulating role of G-quadruplex, we devised an integrating method to perform the algorithm profiling and genome-wide analysis for the dynamic change of genomic G-quadruplex
Yeraldinne Carrasco-Salas et al.
Nucleic acids research, 47(13), 6783-6795 (2019-05-09)
Three-stranded R-loop structures have been associated with genomic instability phenotypes. What underlies their wide-ranging effects on genome stability remains poorly understood. Here we combined biochemical and atomic force microscopy approaches with single molecule R-loop footprinting to demonstrate that R-loops formed
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