MABE866

Anti-Mad2 Antibody, clone AS55-A12

clone AS55-A12, from mouse

• 동의어(들): Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: AS55-A12, monoclonal
• application: IF; WB
• 종 반응성: human
• 기술: immunofluorescence: suitable; western blot: suitable
• citations: 4

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: AS55-A12, monoclonal
• 종 반응성: human
• 기술: immunofluorescence: suitable; western blot: suitable
• 동형: IgG2b
• NCBI 수납 번호: NP_002349
• UniProt 수납 번호: Q13257
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAD2L1(4085)

설명

일반 설명

Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. Mad2 then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease.

면역원

His-tagged recombinant protein corresponding to human Mad2.

애플리케이션

Anti-Mad2 Antibody, clone AS55-A12 is a highly specific mouse monoclonal antibody, that targets MAD2 & has been tested in western blotting & Immunofluorescence.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cell lysate (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad2 in PtK1 cells transfected with Alexa-Mad2 (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cells transfected with pCMV expressing wild-type myc-tagged Mad2, and certain mutations of myc-tagged Mad2 (This mutant does not bind to the core of Mad1/Mad2) (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).

품질

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Mad2 in 10 µg of HEK293 cell lyate.

표적 설명

~23 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable