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Merck
모든 사진(2)

주요 문서

MABE662

Sigma-Aldrich

Anti-TERT Antibody, clone 7D5.2

clone 7D5.2, from mouse

동의어(들):

Telomerase reverse transcriptase, hEST2, hTRT, Telomerase-associated protein 2, Telomerase catalytic subunit, TP2

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

7D5.2, monoclonal

종 반응성

human

기술

immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

human ... TERT(7015)

일반 설명

Telomerase reverse transcriptase (EC 2.7.7.49; UniProt O14746; also known as hEST2, hTRT, Telomerase-associated protein 2, Telomerase catalytic subunit, TP2) is encoded by the TERT (also known as CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TRT) gene (Gene ID 7015) in human. Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase responsible for adding TTAGGG repeats to the chromosome telomere ends. Cells with low or no telomerase expression lose telomere repeats during cell division, eventually resulting in cellular senescence. Most cancer cells, germ cells and embryonic stem cells express high levels of telomerase, thus contributing to pluripotency and immortality. In addition to its telomere maintenance function, telomerase also has a pro-survival role in cellular resistance against DNA damage and ensuing apoptosis. Most cancer cells are highly proliferative and express high levels of nuclear telomerase activity. Studies show that TERT shuttles from the nucleus into mitochondria upon oxidative stress induction in cancer cells. TERT mitochondrial localization helps prevent nuclear DNA damage by decreasing mitochondrial reactive oxygen species (ROS), accounting for high stress resistance especially among the cancer stem cells (CSCs) population. Following exposure to H2O2 or gamma-irradiation, cancer cells capable of excluding TERT from the nucleus display little or no DNA damage, while TERT nuclear retainment results in high DNA damage.

특이성

Clone 7D5.2 targets a sequence toward the end of the C-terminal extension (CTE) region present in spliced isoforms 1 and 3, but not in isoforms 2 and 4 of human TERT reported by UniProt (O14746).

면역원

KLH-conjugated linear peptide corresponding to a sequence toward the end of the C-terminal extension (CTE) region of human TERT.

애플리케이션

Anti-TERT, clone 7D5.2 , Cat. No. MABE662, is a highly specific mouse monoclonal antibody that targets hTERT and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TERT in human cerebral cortex, kidney, and tonsil tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected TERT in 10 µg of Jurkat cell lysate.

품질

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TERT in 10 µg of PC3 human prostate cancer cell lysate.

표적 설명

~140 kDa observed. 127.0/120.0 kDa (human isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).

결합

Replaces: MABE14

물리적 형태

Format: Purified
Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

기타 정보

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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