MABE365

Anti-acetyl PARP1 Antibody, clone E4

clone E4, from mouse

• 동의어(들): Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: E4, monoclonal
• application: WB
• 종 반응성: human
• 기술: western blot: suitable
• citations: 1

속성

• 생물학적 소스: mouse
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: E4, monoclonal
• 종 반응성: human
• 기술: western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_001609
• UniProt 수납 번호: P09874
• 배송 상태: wet ice
• 타겟 번역 후 변형: acetylation (not specified)
• 유전자 정보: human ... PARP1(142)

설명

일반 설명

Poly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.

면역원

Linear peptide corresponding to acetylated human PARP1, short fragment.

애플리케이션

Anti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).

품질

Evaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.

표적 설명

~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Acetylated PARP1 fragment (373-529) purified protein lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable