MABE308

Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

ascites fluid, clone 1RC-1C10, from mouse

• 동의어(들): E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein, Nuclear protein 95, Nuclear zinc finger protein Np95, HuNp95, RING finger protein 106, Transcription factor ICBP90, Ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 1RC-1C10, monoclonal
• 종 반응성: human
• 기술: ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_001041666
• UniProt 수납 번호: Q96T88
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... UHRF1(29128)

설명

일반 설명

Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.

면역원

Recombinant protein corresponding to human ICBP90.

애플리케이션

Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10 is a Mouse Monoclonal Antibody for detection of ICBP90/UHRF1 also known as E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein & has been validated in WB, ChIP, ICC & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in HeLa and MOLT-4 cell lysates (Hopfner, R., et al. (2001). 266(1-2)15-23.).

Immunocytochemistry Analyisis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in ICC (Hopfner, R., et al. (2001). Gene. 266(1-2)15-23.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in human appendix and human breast carcinoma tissues (Hopfner, R., et al. (2000). Cancer Res. 60(1):121-128.).

Chromatin Immunoprecipitation Analyis: A representative lot from an independent laboratory immunoprecipitated ICB90/UHRF1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19). 8355-8365.)

품질

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected ICBP90/UHRF1 in 10 µg of HeLa cell lysate.

표적 설명

~90 kDa observed

물리적 형태

Mouse monoclonal IgG1 ascites with 0.05% sodium azide.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
HeLa cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable