MABE2028

Anti-FAN1 Antibody, clone 1A11-2-A

• 동의어(들): EC:3.1.21, FANCD2/FANCI-associated nuclease 1, Fanconi-associated nuclease 1, Myotubularin-related protein 15, hFAN1
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1A11-2-A, monoclonal
• 분자량: calculated mol wt 114.23 kDa; observed mol wt ~115 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: C-terminal
• 단백질 ID 수납 번호: NP_001139566
• UniProt 수납 번호: Q9Y2M0
• 저장 온도: 2-8°C
• 유전자 정보: human ... FAN1(22909)

설명

특이성

Clone 1A11-2-A is a mouse monoclonal antibody that detects Fanconi-associated nuclease 1 (FAN1). It targets an epitope within the C-terminal region.

면역원

His-tagged recombinant fragment corresponding to 147 amino acids from the C-terminal region of human Fanconi-associated nuclease 1 (FAN1).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type HeLa cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FAN1 in lysate from wild-type HeLa cells, but not in lysate form FAN1 knockout HeLa cells.

Tested Applications

Proximity Ligation Assay: A representative lot detected FAN1 in Proximity Ligation Assay (Porro, A., et al. (2021). Sci Adv. 7(31):eabf7906).

Western Blotting Analysis: A representative lot detected FAN1 in Western Blotting applications (Porro, A., et al. (2021). Sci Adv. 7(31):eabf7906).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Fanconi-associated nuclease 1 (UniProt: Q9Y2M0; also known as EC:3.1.21, FANCD2/FANCI-associated nuclease 1, hFAN1, Myotubularin-related protein 15) is encoded by the FAN1 (also known as KIAA1018, MTMR15) gene (Gene ID: 22909) in human. FAN1 is a nuclease that is required for the repair of DNA interstrand cross-links (ICL). It is recruited to the sites of DNA damage by monoubiquitinated FANCD2, an effector of ATR signaling. It acts as a 5′-3′ exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. It is specifically involved in repair of ICL-induced DNA breaks and is required for efficient homologous recombination. However, it is not involved in DNA double-strand breaks resection. It is shown to hydrolytically remove 5′-nucleotides successively from the 3′-hydroxy termini of 3′-hydroxy-terminated oligonucleotides. FAN1 is reported to interact with MLH1 via two adjacent MLH1-binding sites, referred to as MLH1-interacting protein (MIP) box and MLH1-interacting motif (MIM). This interaction is regulated during the cell cycle and is inhibited in G2/M phase following CDK1/2-mediated phosphorylation of serine 126 within the MIP box of FAN1. Disruption of the FAN1-MLH1 interaction is reported to cause hypersensitivity to DNA cross-linking agents and defective repair of slipped-CAG/CTG repeats. Two isoforms of FAN1 have been described that are produced by alternative splicing. (Ref.: Porro, A., et al. (2021). , Sci. Adv. 7; eabf7906; Thongthip, S., et al. (2016). Genes & Dev. 2016. 30: 645-659; Wang, R., et al. (2014). Science. 346(6213); 1127-1130; Mackay, C., et al. (2010). Cell. 142(1); 65-76).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1