MABE2008

Anti-p-Histone H2A.X (Ser139) Recombinant Antibody, clone C6B

recombinant, expressed in Alpaca

• 동의어(들): H2a/x, Histone H2A.X, Histone H2AX
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: alpaca
• Quality Level: 200
• 재조합: expressed in Alpaca
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: C6B, monoclonal
• 분자량: calculated mol wt 15.14 kDa
• 정제법: immobilized metal affinity chromatography (IMAC)
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• 에피토프 서열: C-terminal
• 단백질 ID 수납 번호: NP_002096
• UniProt 수납 번호: P16104
• 저장 온도: -10 to -25°C
• 유전자 정보: human ... H2AX(3014)

설명

특이성

Clone C6B is an E. coli expressed recombinant monoclonal Alpaca antibody that specifically detects Histone H2A.X phosphorylated on serine 139.

면역원

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 139 from the C-terminal region of human Histone H2A.X.

애플리케이션

Quality Control Testing

Evaluated by Immunocytochemistry in MCF7 cells.

Immunocytochemistry Analysis: 2 ng/mL from a representative lot detected phospho-Histone H2AX (Ser139) in MCF7 cells treated with 25 M etoposide for 24 h.

Tested Applications

Western Blotting Analysis: A representative lot detected Histone H2AX in Western Blotting application (Moeglin, E., et al. (2021). Cancers (Basel). 13(13): 3317).

Immunocytochemistry Analysis: A representative lot detected Histone H2AX in Immunocytochemistry application (Moeglin, E., et al. (2021). Cancers (Basel). 13(13): 3317).

ELISA Analysis: A representative lot detected Histone H2AX in ELISA application (Moeglin, E., et al. (2021). Cancers (Basel). 13(13): 3317).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Histone H2AX (UniProt: P16104; also known as H2a/x, Histone H2A.X) is encoded by the H2AX (also known as H2AFX) gene (Gene ID: 3014) in human. The histone H2A.X protein is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR at the fourth residue from the carboxy-terminus (serine 139 in mammalian H2A.X) in response to DNA double-strand breaks (DSBs). Histone H2AX plays a crucial role in the maintenance of genome stability and the DNA damage response (DDR). H2AX is ubiquitously expressed in almost all eukaryotic cells and is primarily localized in the cell nucleus, where it plays a crucial role in the packaging and organization of DNA within chromatin. Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage. H2A.X phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation. Phosphorylation of H2A.X occurs irrespective of the cause of the DNA DSBs and phospho-H2A.X has been observed in response to environmental stresses that result in DSBs as well as programmed cellular events, including DNA rearrangement and apoptosis. Histone H2AX phosphorylated at serine 139 (gamma H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. The small size and great affinity for gamma H2AX, allows for efficient electroporation of this C6B nanobody in living cells and, detection of gamma H2AX foci in non-fixed live cells in real time imaging. (Ref.: Moeglin, E., et al. (2021). Cancers (Basel). 13(13):3317).

물리적 형태

Purified E. coli expressed recombinant monoclonal Alpaca antibody in PBS without preservatives with 10% glycerol.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable