MABE2005

Anti-RNF4 Antibody, clone 11-3.1

• 동의어(들): E3 ubiquitin-protein ligase RNF4, Protein SNURF, RING finger protein 4, Small nuclear ring finger protein
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 11-3.1, monoclonal
• 분자량: calculated mol wt 21.32 kDa; observed mol wt ~32 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2bκ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_001171938
• UniProt 수납 번호: P78317
• 저장 온도: 2-8°C
• 유전자 정보: human ... RNF4(6047)

설명

특이성

Clone 11-3.1 is a mouse monoclonal antibody that specifically detects recombinant human RNF4 protein.

면역원

GST-tagged, recombinant human RNF4 protein.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

E3 ubiquitin-protein ligase RNF4 (UniProt: P78317; also known as RING finger protein 4, EC:2.3.2.27, Small nuclear ring finger protein, Protein SNURF) is encoded by the RNF4 (also known as SNURF) gene (Gene ID: 6047) in human. RNF4 gene encodes ubiquitin E3 ligase, an enzyme widely expressed in many tissues. RNF4 is mapped to human chromosome 4p16.3. The protein contains a RING-type zinc finger domain and around four small ubiquitin-related modifier (SUMO)-interaction motifs. The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates and the SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate. RNF4 is predominantly localized in the nucleus but can also be found in the cytoplasm. This dual localization allows it to function in multiple cellular compartments, dealing with SUMOylated substrates wherever they are located. The movement between these compartments can be regulated by specific signals or stress conditions. RNF4 is broadly expressed across various tissues, with notable levels in tissues that have high cellular turnover or are particularly responsive to stress, such as in immune cells and testis. It is involved in DNA repair, in transcription and chromatin regulation. It is also associated with the process of hematopoiesis. RNF4 is implicated in acute promyelocytic leukemia, acute myeloid leukemia, Fanconi anemia and aplastic anemia. Its role in maintaining protein homeostasis and responding to cellular stress makes it a key player in health and disease, with its dysfunction being linked to cancer, neurodegeneration, and developmental issues. Understanding the mechanisms regulating RNF4 activity and localization can provide insights into therapeutic strategies for these diseases. (Ref.: Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8. (PMID: 24695317).

물리적 형태

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable