MABE1985

Anti-Gemin5 Antibody, clone 1E12

• 동의어(들): Gem-associated protein 5
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1E12, monoclonal
• 분자량: calculated mol wt 168.59 kDa; observed mol wt ~170 kDa
• 정제법: using protein G
• 종 반응성: human, human, monkey
• 종 반응성(상동성에 의해 예측): porcine
• 포장: antibody small pack of 100 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1
• 에피토프 서열: C-terminal
• 단백질 ID 수납 번호: NP_001239085
• UniProt 수납 번호: Q8TEQ6
• 배송 상태: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GEMIN5(25929)

설명

일반 설명

Gem-associated protein 5 (UniProt: Q8TEQ6; also known as Gemin5) is encoded by the GEMIN5 gene (Gene ID: 25929) in human. Survival of motor neurons (SMN) protein is known to form a stable complex with a group of proteins named gemins. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs) and thereby plays an important role in the splicing of cellular pre-mRNAs. Gemin5, a highly conserved, multi-functional protein, is a component of the core SMN complex and is found in the nucleoplasm and nuclear bodies known as Gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. It is also abundantly present in the cytoplasm. It plays a role in capturing snRNA into SMN complexes in the cytoplasm for transport into the nucleus. However, in the nucleus it is easily detached from the SMN-Gemin complex. Gemin5 is reported to physically bind to snRNA via a specific AU5-6 sequence located within the highly conserved Sm site and is flanked by a short stem loop that assists in delivery to the SMN complex. The specific snRNP code helps Gemin5 in distinguishing these snRNAs from other forms of cellular RNAs. Gemin5 contains 13 WD repeat domains that mediate its binding to U1 and U4 snRNA. WD domains are also reported to mediate binding to the 7-methylguanosine cap that is found on mRNA and snRNA molecules. Mutations in GEMIN5 gene are known to cause a distinct neurological cerebellar ataxia syndrome, through altered snRNP complex assembly. (Ref.: Kour, S., et al. (2021). Nat. Commun. 12; Article 2558; Hao, L., et al. (2007). BMC Cell Biol. 8:28).

특이성

Clone 1E12 is a mouse monoclonal antibody that detects Gem-associated protein 5 (Gemin5). It targets an epitope within the C-terminal region.

면역원

His-tagged recombinant fragment corresponding to 292 amino acids form the C-terminal region of human Gem-associated protein 5 (Gemin5).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in COS-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in HeLa cell lysate.

Immunocytochemistry Analysis: A representative lot detected Gemin5 in Immunocytochemistry application (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28; Schilling, M., et al. (2021). Cell Rep. 35(12); 109277).

Western Blotting Analysis: A representative lot detected Gemin5 in Western Blotting applications (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable