MABE1154

Anti-Fibrillarin Antibody, clone 38F3

clone 38F3, from mouse

• 동의어(들): Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 38F3, monoclonal
• 종 반응성: human, rat, yeast, mouse, Drosophila
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_010270.1
• UniProt 수납 번호: P15646
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FBL(2091)

설명

일반 설명

rRNA 2′-O-methyltransferase fibrillarin (UniProt: P15646; also known as Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1) is encoded by the NOP1 (also known as LOT3, YDL014W, D2870) gene (Gene ID: 851548) in yeast. Fibrillarin is a nucleolar protein that is highly conserved throughout evolution. Human fibrillarin is 67% identical to its yeast homolog Nop1p. Fibrillarin is a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs) that direct 2 -O-methylation of rRNA and is also involved in other aspects of rRNA processing. Unlike snRNAs, which have a cytoplasmic phase of maturation, snoRNAs are restricted to the nucleus. Fibrillarin is essential for yeast viability and fibrillarin knockout is shown to be lethal in S. cerevisiae; however, expression of human or Xenopus fibrillarin overcome this lethality. The first 80 amino acids from the N terminal region of fibrillarin contain multiple RGG, or arginine-glycine-glycine (GAR). The remaining ~240 amino acids consist of the so called fibrillarin domain. NOP1 depleted strains of yeast are greatly impaired in the production of cytoplasmic ribosomes, and they have a reduced level of rRNA. Patients with scleroderma are reported to have strong circulating autoantibodies to fibrillarin. (Ref.: Tollervey, D et al. (1991). EMBO J. 10(3):573-83; Newton, K et al. (2003). Mol. Cell. Biol. 23(23): 8519-8527).

특이성

Clone 38F3 specifically detects Fibrillarin/Nop1p in multiple mammalian species and in yeast.

면역원

Yeast nuclear extract

애플리케이션

Anti-Fibrillarin, clone 38F3, Cat. No. MABE1154, is a highly specific mouse monoclonal antibody that targets Fibrillarin/Nop1p and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected Fibrillarin in human SH-SY5Y cells (Courtesy of Dr Gerry Shaw).

Immunocytochemistry Analysis: A representative lot detected Fibrillarin in Immunocytochemistry applications (Newton, K., et. al. (2003). Mol. Cell. Biol. 23(23):8519-27).

Research Category
Epigenetics & Nuclear Function

품질

Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibrillarin in 10 µg of HeLa cell lysates.

표적 설명

~34 kDa observed; 34.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing PBS with 0.03% sodium azide with 50% glycerol.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2