MABE1151
Anti-OASIS (CREB3L1) Antibody, clone 10H1
clone 10H1, from mouse
동의어(들):
Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS
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모든 사진(1)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
10H1, monoclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
포장
antibody small pack of 25 μg
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG2aκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... CREB3L1(90993)
일반 설명
Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in
unfolded protein response (UPR). It binds the DNA consensus sequence 5′-GTGXGCXGC-3′. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.
unfolded protein response (UPR). It binds the DNA consensus sequence 5′-GTGXGCXGC-3′. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.
특이성
Clone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region.
면역원
KLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1).
애플리케이션
Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues.
Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).
Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).
Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).
Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).
품질
Evaluated by Immunohistochemistry in human prostate cancer tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.
표적 설명
57.00 kDa calculated.
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Daniele Pittari et al.
Frontiers in cell and developmental biology, 10, 986997-986997 (2022-11-01)
Upon progesterone stimulation, Endometrial Stromal Cells (EnSCs) undergo a differentiation program into secretory cells (decidualization) to release in abundance factors crucial for embryo implantation. We previously demonstrated that decidualization requires massive reshaping of the secretory pathway and, in particular, of
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