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Merck
모든 사진(1)

주요 문서

MABE1123

Sigma-Aldrich

Anti-XPB Antibody, clone 15TF2-1B3

ascites fluid, clone 15TF2-1B3, from mouse

동의어(들):

TFIIH basal transcription factor complex helicase XPB subunit, Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

15TF2-1B3, monoclonal

종 반응성

human

기술

immunocytochemistry: suitable
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... ERCC3(2071)

일반 설명

Transcription factor II human (TFIIH) basal transcription factor complex helicase XPB subunit (EC 3.6.4.12; UniProt P19447; also known as BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, Basic transcription factor 2 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein) is encoded by the ERCC3 (also known as BTF2, GTF2H, RAD25, TFIIH, XPB) gene (Gene ID 2071) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by TFIIH. The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.

면역원

Epitope: N-terminus
Recombinant protein corresponding to human XPB.

애플리케이션

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-XPB Antibody, clone 15TF2-1B3 is validated for use in Western Blotting, Immunocytochemistry for the detection of XPB.
Western Blotting Analysis: A representative lot detected Xpb in murine embryonic fibroblasts (MEFs) and HeLa cells, as well as in transgenic animal-derived MEFs expressing Xpb lacking last 43 C-terminal amino acids (Andressoo, J.O., et al. (2009). Mol Cell Biol.29(5):1276-290).
Western Blotting Analysis: A representative lot detected endougenous as well as exogenously expressed Xpb in both U2OS17 whole cell lysate and in THIIF p62 subunit immunoprecipitate (Ziani, S., et al. (2014). J Cell Biol.;206(5):589-598).
Western Blotting Analysis: A representative lot detected Xpb in THIIF TTDA subunit immunoprecipitate (Giglia-Mari,G., et al. (2006). PLoS Biol. 4(6): e156).
Immunocytochemistry Analysis: A representative lot detected XPB recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).

품질

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPB in 10 µg of HeLa nuclear extract.

표적 설명

~85 kDa observed. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin.
Ziani, S; Nagy, Z; Alekseev, S; Soutoglou, E; Egly, JM; Coin, F
The Journal of cell biology null
An Xpb mouse model for combined xeroderma pigmentosum and cockayne syndrome reveals progeroid features upon further attenuation of DNA repair.
Andressoo, JO; Weeda, G; de Wit, J; Mitchell, JR; Beems, RB; van Steeg, H; van der Horst et al.
Molecular and cellular biology null
Sylvain Geny et al.
Methods in molecular biology (Clifton, N.J.), 2247, 39-57 (2020-12-11)
Macromolecular complexes govern the majority of biological processes and are of great biomedical relevance as factors that perturb interaction networks underlie a number of diseases, and inhibition of protein-protein interactions is a common strategy in drug discovery. Genome editing technologies
Giuseppina Giglia-Mari et al.
PLoS biology, 4(6), e156-e156 (2006-05-04)
Transcription/repair factor IIH (TFIIH) is essential for RNA polymerase II transcription and nucleotide excision repair (NER). This multi-subunit complex consists of ten polypeptides, including the recently identified small 8-kDa trichothiodystrophy group A (TTDA)/ hTFB5 protein. Patients belonging to the rare
Sergey Alekseev et al.
Chemistry & biology, 21(3), 398-407 (2014-02-11)
Nucleotide excision repair (NER) removes DNA lesions resulting from exposure to UV irradiation or chemical agents such as platinum-based drugs used as anticancer molecules. Pharmacological inhibition of NER is expected to enhance chemosensitivity but nontoxic NER inhibitors are rare. Using

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