MABE1116

Anti-DNAJB1/Hdj1 Antibody, clone J25

clone J25, from mouse

• 동의어(들): Hdj1, DnaJ homolog subfamily B member 1, DNAJB1, hDj-1, Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: J25, monoclonal
• 종 반응성: rat, mouse, human
• 종 반응성(상동성에 의해 예측): bovine (based on 100% sequence homology)
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_001028935
• UniProt 수납 번호: Q3MI00
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DNAJB1(3337)

설명

일반 설명

DnaJ homolog subfamily B member 1 (UniProt Q3MI00) is encoded by the DNAJB1 gene (Gene ID 538426) in bovine species. Heat shock proteins (Hsps) are classified into six major families based on their relative molecular mass, Hsp110/hspH, Hsp90/HspC, Hsp70/HspA, Hsp60/HspD, Hsp40/DnaJ, and small heat shock proteins sHsp/HspB. Hsp40/DnaJB1 belongs to the Hsp40/DnaJ subfamily of heat shock proteins. Hsp40 acts as a co-chaperone with Hsp70 to facilitate protein folding, transport, and degradation. MK5 is reported to stimulate the ATP hydrolyse activity of Hsp40/Hsp70 complex by phosphorylating Hsp40/DnaJB1 C-terminal serine residues Ser149, Ser151, Ser171, resulting in an enhancement of Hsp40/DnaJB1-dependent suppression of heat shock factor 1-driven transcription. DnaJB1 is also shown to interact with MDM2 and prevent MDM2-mediated ubiquitination and proteasomal degradation of p53. DnaJB1 depletion promotes cancer cell growth as a result of p53 pathway inactivation.

면역원

Purified bovine DNAJB1.

애플리케이션

Immunocytochemistry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Flow Cytometry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Western Blotting Analysis: 0.05-0.5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).

This Anti-DNAJB1/Hdj1 Antibody, clone J25 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of DNAJB1/Hdj1.

품질

Evaluated by Western Blotting in human K562 erythroblastoma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected heat shock-induced DNAJB1/Hdj-1 upregulation in human K-562 erythroleukemia cells.

표적 설명

~38 kDa observed

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable