MABE1114

Anti-CENP-C Antibody, clone AL61A

clone AL61A, from mouse

• 동의어(들): Centromere protein C, CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: AL61A, monoclonal
• application: ICC; IP
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: AL61A, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001803
• UniProt 수납 번호: Q03188
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CENPC(1060)

설명

일반 설명

Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to casue mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.

특이성

Expected to react with both spliced isoforms of human CENP-C reported by UniProt (Q03188).

면역원

Epitope: N-terminal.

GST-tagged recombinant human CENP-C N-terminal fragment.

애플리케이션

Anti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.

Immunoprecipitation Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunoprecipitated CENP-C from HeLa nuclear extract (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant detected nuclear CENP-C puncta by fluorescent immunocytochemistry staining of permeabilized HeLa and Jurkat cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunostained CENP-C-transfected, but not ZBTB32-transfected, HEK293T cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).

품질

Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected CENP-C in Jurkat cells.

표적 설명

106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable