MABE1066

Anti-SETDB1 Antibody, clone F0838

clone F0838, from mouse

• 동의어(들): Histone-lysine N-methyltransferase SETDB1, EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: F0838, monoclonal
• 종 반응성: mouse
• 포장: antibody small pack of 25 μg
• 기술: western blot: suitable
• 동형: IgG1κ
• UniProt 수납 번호: O88974
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Setdb1(84505)

설명

일반 설명

Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in human. SETDB1 is ubiquitously expressed, but a strong expression is observed in liver and testis. It is a histone methyltransferase that specifically trimethylates Histone 3 Lysine 9 (H3K9). H3K9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB1 is reported to inhibit adipogenesis. It has also been shown that H3K4/H3K9me3 bivalent chromatin signature keeps expression of Cebpa and Pparg genes very low. Depletion of SETDB1 can induce adipogenesis in mesenchymal stem cells and in preadipocytes. SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and one MBD domain (aa 611-682). It also has a SET domain (aa 820-1282), a pre-SET (aa 744-817), and a post-SET domain (aa 1291-1307). The pre-SET, SET and post-SET domains are required for its methyltransferase activity. In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. (Ref.: Matsumura, Y., et al., (2015), Mol. Cell 60(4); 584- 596).

특이성

Clone F0838 recognizes SETDB1 in murine embryonic stem cells. It targets an epitope with in 213 amino acids from the N-terminal half of isoform 1 and 7.

면역원

His-tagged recombinant fragment corresponding to 213 amino acids from the N-terminal half of murine Histone-lysine N-methyltransferase SETDB1.

애플리케이션

Anti-SETDB1, clone F0838, Cat. No. MABE1066, is a highly specific mouse monoclonal antibody that detects SETDB1 and has been tested for use in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected SETDB1 in Western Blotting applications (Matsumura, Y, et. al. (2015). Mol Cell. 60(4):584-96).

품질

Evaluated by Western Blotting in 129/S6/SvEv mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SETDB1 in 129/S6/SvEv mouse embryonic stem cell lysate.

표적 설명

~160 and 180 kDa observed; 144.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.